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EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line

Mouse

Synonym(s):

JK1 Feeder Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

Product Name

EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line, Immortalized Mouse Feeder Cell Line

biological source

mouse

manufacturer/tradename

EmbryoMax®

technique(s)

cell culture | stem cell: suitable

shipped in

ambient

General description

JK1 is an immortalized CD34+SMA+ cell line derived from spontaneous transformation of C57/BL6 mouse testicular stromal cells. JK1 cells are able to support the long-term culture of a variety of primitive cells including multi-potent adult spermatogonial-derived stem cells, pluripotent murine embyronic stem cells and embyronic germ cells derived from primordial germ cells. Adult spermatogonial stem and progenitor cells (SPCs) cultured on JK1 feeder cells express key features of germ line stem cells including expression of PLZF, DAZL, and GCNA1. JK1 feeder cells has maintained its capacity for promoting stem cell self-renewal even after serial passaging for over one year.

Cell Line Description

Fibroblast Cells

Application

Immortalized Mouse Feeder Cell Line
Research Category
Stem Cell Research
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Quality

• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Storage and Stability

EmbryoMax JK1 Murine Testicular Stromal Feeder Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

SCC169:

Certificates of Analysis (COA)

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