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SCC112

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FLAG-SNAP-hTERT expressing HeLa Cell Line

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203

Product Name

FLAG-SNAP-hTERT expressing HeLa Cell Line, FLAG-SNAP-hTERT expressing HeLa Cell Line is a useful tool for studying telomerase biology and enables purification and visualization of endogenous telomerase reverse transcriptase (TERT).

biological source

human

Quality Level

100

shipped in

ambient

General description

Telomerase play a critical role in regulating proliferation and senescence in normal somatic cells as well as most cancer cells. In humans, the telomerase reverse transcriptase (hTERT) is a lowly expressed protein with only several hundred molecules per cell. The low expression has made it difficult to detect, purify and study TERT activity from the endogenous loci.
To facilitate reliable detection of the endogenous TERT protein, a FLAG-SNAP tag was fused to the N-terminus of the TERT locus in HeLa cells. The FLAG-SNAP tag does not impair the function of the endogenous hTERT protein. Efficient immunopurification (IP) of the active telomerase ribonucleoprotein complexes may be accomplished by using the well-characterized FLAG antibody. The SNAP tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.

Reference:
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.

Cell Line Description

Cancer Cells

Application

Research Category
Cell Culture
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Cells are positive for HPV-18 by PCR.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCC112:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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