Skip to Content
Merck
All Photos(7)

Key Documents

Safety Information

SCC107

Sigma-Aldrich

CR4 Human Colorectal Cancer Cell Line

Human

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

CR4 Human Colorectal Cancer Cell Line, CR4 is a unique, cancer stem cell (CSC) enriched, human colorectal cancer cell line useful for CSC-targeted drug development and research focused on cancer cell and CSC biology.

biological source

human

Quality Level

General description

CR4 is a novel CIC-enriched, highly tumorigenic, clonogenic and 3D spheroid-forming colon cancer cell line derived from the liver metastasis of a male patient with stage 4 colon cancer (1). Primary tumor cells were established on type I collagen coated flasks in serum-free stem cell media (1). Holoclones, small stem-like cells that are densely packed, were selectively subcloned from the heterogeneous culture and propagated. Cells were then passaged as 3D spheroids and in NOD/SCID mice xenografts leading to the eventual establishment of the CR4 human colorectal cancer cell line.
Majority of CR4 cells express high but variable levels of common cancer stem cell markers including CD44, CD166, EpCAM, and Lgr51. CR4 cell line has the G12V mutation on KRAS exon 2 (Botchkina GI, communications).

Reference:
1. Rowehl RA, Burke S, Bialkowska AB, Pettet DW III, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, Ojima I., Botchkina GI (2014) PLOS 9(6): e99091.
Colorectal cancer (CRC) is the 2nd highest cause of cancer death in the United States. Increasing evidence suggests that a small population of cancer-initiating cells (CICs) or cancer stem cells (CSCs) are responsible for tumorigenesis and the exceptional drug resistance to anti-cancer therapies.

Quality

• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, Herpesvirus type 7, 8 and HIV-1 & 2 viruses by PCR
• Cells are positive for herpesvirus type 6 by PCR analysis.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCC107:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service