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Safety Information

S8005H

Sigma-Aldrich

CpGenome 5-hmC DNA Standard

CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.84

Quality Level

100

species reactivity

human

manufacturer/tradename

CpGenome
Upstate®

shipped in

dry ice

Related Categories

General description

CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.

Materials Provided:

One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.

Validation:

Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.

Application

Research Category
Epigenetics & Nuclear Function

Physical form

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Storage and Stability

Recommended Storage: Stable for 6 months at -20°C from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S8005H:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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