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Safety Information

NA46

Sigma-Aldrich

Anti-p300 Mouse mAb (NM-11)

liquid, clone NM-11, Calbiochem®

Synonym(s):

Anti-p300 antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

NM-11, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, rat

should not react with

mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EP300(2033)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Immunogen

Epitope: within amino acids 2071-2091 near the CBP C-terminus
Human
recombinant, human p300 protein

Application

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A431, HeLa, or ML-1 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

NA46-MG:
NA46-100UG:
NA46-ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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