MABS1704

Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1

clone 10C1.1, from mouse

• Synonym(s): ATP-sensitive inward rectifier potassium channel 11, IKATP, Inward rectifier K(+) channel Kir6.2, Potassium channel, inwardly rectifying subfamily J member 11
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10C1.1, monoclonal
• species reactivity: human, rat
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001159762
• UniProt accession no.: Q14654
• target post-translational modification: unmodified
• Gene Information: human ... KCNJ11(3767)

Description

General description

ATP-sensitive inward rectifier potassium channel 11 (UniProt Q14654; also known as IKATP, Inward rectifier K(+) channel Kir6.2, Potassium channel, inwardly rectifying subfamily J member 11) is encoded by the KCNJ11 (also known as BIR, HHF2, MODY13, PNDM, TNDM3) gene (Gene ID 3767) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. ATP-sensitive potassium (K-ATP) channels are hetero-octamers composed of pore-forming Kir6.x (6.1 or 6.2) subunits and sulfonylurea receptor (SUR1 or SUR2) regulatory subunits. K-ATP channels are regulated by intracellular ATP and ADP concentrations and are highly expressed in cardiomyocytes, where they play crucial roles in ischemic preconditioning (IPC). Potassium channel openers mimic the cardioprotection, while K-ATP channel blocker or Kir6.2-knockout is shown to abolish the effect of IPC in mice. Human Kir6.2 is a 390-amino acid multi-pass membrane (a.a. 69-93, 117-128, 145-166) protein that contains two small extraceullar regions (a.a. 94-116 and 136-144) and a pore-forming domain (a.a. 117-128), having both its N- and C-terminal exposed intracellularly (a.a. 1-68 and a.a. 167-390).

Specificity

Clone 10C1.1 targets human Kir6.2 N-terminal cytoplasmic region not present in the alternatively spliced isoform 2.

Immunogen

Epitope: N-terminal cytoplasmic domain.

KLH-conjugated linear peptide corresponding an N-terminal cytoplasmic domain sequence of human potassium channel Kir6.2.

Application

Research Category
Signaling

Research Sub Category
Ion Channels & Transporters

This Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1 is validated for use in Western Blotting for the detection of Potassium Channel Kir6.2.

Western Blotting Analysis: A 1:1000 dilution from a representative lot detected potassium channel Kir6.2 in 10 µg of rat pancreas tissue lysate.

Quality

Evaluated by Western Blotting in human pancreas tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected potassium channel Kir6.2 in 10 µg of human pancreas tissue lysate.

Target description

~45 kDa observed. 43.54 kDa (human) and 43.61 kDa (rat) calculated.

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable