MABS1273
Anti-Thrombomodulin Antibody, clone 411-201B
clone 411-201B, from rat
Synonym(s):
Thrombomodulin, CD141, Fetomodulin, SnoRNA MBII-339, TM
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
411-201B, monoclonal
species reactivity
human, mouse
technique(s)
ELISA: suitable
electron microscopy: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... THBD(7056)
General description
Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that functions to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombin′s procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577).
Immunogen
Epitope: Extracellular domain.
Normal mouse lung homogenate.
Application
Anti-Thrombomodulin Antibody, clone 411-201B is an antibody against Thrombomodulin for use in Immunohistochemistry (Paraffin), Electron Microscopy, Immunoprecipitation, Western Blotting, ELISA.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue.
Electron Microscopy: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblast (NMLF) lysates, but not from thrombomodulin-depleted NMLF lysates by performing IP with clone 273-34A (Cat. No. MABS1245) prior to IP with clone 411-201B (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Western Blotting Analysis: A representative lot detected thrombomodulin (P112) in the normal mouse lung fibroblast (NMLF) immunoprecipitates obtained with either clone 411-201B or clone 273-34A (Cat. No. MABS1245) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunohistochemistry Analysis: Representative lots and clone 273-34A (Cat. No. MABS1245) showed similar staining pattern using both frozen and paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Rorvik, M.C., et al. (1988). J. Histochem. Cytochem. 36(7):741-749; Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
ELISA Analysis: Representative lots were used as the detection antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 273-34A (Cat. No. MABS1245) as the capture antibody and an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay employing 125I-labeled clone 411-201B without an enzyme-conjugated secondary antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).
Electron Microscopy: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblast (NMLF) lysates, but not from thrombomodulin-depleted NMLF lysates by performing IP with clone 273-34A (Cat. No. MABS1245) prior to IP with clone 411-201B (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Western Blotting Analysis: A representative lot detected thrombomodulin (P112) in the normal mouse lung fibroblast (NMLF) immunoprecipitates obtained with either clone 411-201B or clone 273-34A (Cat. No. MABS1245) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunohistochemistry Analysis: Representative lots and clone 273-34A (Cat. No. MABS1245) showed similar staining pattern using both frozen and paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Rorvik, M.C., et al. (1988). J. Histochem. Cytochem. 36(7):741-749; Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
ELISA Analysis: Representative lots were used as the detection antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 273-34A (Cat. No. MABS1245) as the capture antibody and an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay employing 125I-labeled clone 411-201B without an enzyme-conjugated secondary antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).
Research Category
Signaling
Signaling
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by Immunohistochemistry in mouse lung tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Thrombomodulin in mouse lung tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Thrombomodulin in mouse lung tissue.
Target description
61.87 kDa calculated.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MABS1273:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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