Skip to Content
Merck
All Photos(2)

Key Documents

Safety Information

MABN730

Sigma-Aldrich

Anti-Rab10 Antibody, clone 4E2

ascites fluid, clone 4E2, from mouse

Synonym(s):

RAB10, Ras-related protein Rab-10, Rab10

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4E2, monoclonal

species reactivity

human, mouse

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAB10(10890)

General description

Ras related protein Rab-10 (Rab10) is encoded by the gene named RAB10 and is member of the small GTPase family of Rab proteins. Rab proteins play critical roles in intracellular trafficking from the formation of vesicles to their fusion with membranes. Specifically Rab10 is involved in regulating RME-1 in endocytic recycling as well as endoplasmic reticulum structural dynamics and morphology, and Rab10 also appears to be critical to formation and correct placement of proteins in such critical cellular structures as the basement membranes of during organ morphogenesis and basement membrane remodeling during development. Rab10 localizes to internal membrane structures like the endoplasmic reticulum and Golgi apparatus but in certain cells it can be found associated with cilia and other cellular structures as well. EMD-Millipore’s Anti-Rab10 mouse monoclonal antibody has been tested in western blot against human HeLa and mouse NIH3T3 cell lysates and in fluorescent immunocytochemistry on LOVO cells in culture.

Immunogen

Purified recombinant fragment of human Rab10 expressed in E. Coli.

Application

Detect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Rab10 in LOVO cells.

Optimal working dilutions must be determined by end user.



Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in HeLa and NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.

Target description

~25 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa and NIH/3T3 cell lysates

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABN730:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Natalie Connor-Robson et al.
Neurobiology of disease, 127, 512-526 (2019-04-08)
Mutations in LRRK2 are the most common cause of autosomal dominant Parkinson's disease, and the relevance of LRRK2 to the sporadic form of the disease is becoming ever more apparent. It is therefore essential that studies are conducted to improve
Daniel R Gulbranson et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(39), E8224-E8233 (2017-09-13)
Rab GTPases are switched from their GDP-bound inactive conformation to a GTP-bound active state by guanine nucleotide exchange factors (GEFs). The first putative GEFs isolated for Rabs are RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) and its yeast homolog DSS4

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service