MABF455

Anti-CD80 (B7-1) Antibody (mouse), PE, clone 16-10A1

clone 16-10A1, 0.2 mg/mL, from hamster(Armenian)

• Synonym(s): T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: hamster (Armenian)
• Quality Level: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 16-10A1, monoclonal
• species reactivity: mouse
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG
• UniProt accession no.: Q00609
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd80(12519)

Description

General description

The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This armenian hamster monoclonal antibody Anti-CD80 (B7-1) (mouse), PE, clone 16-10A1 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD80 (B7-1).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD80in one million C57Bl/6 splenocytes.

Physical form

Purified Armenian Hamster Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable