MABF3230

Anti-CD70 Antibody, clone FR70

• Synonym(s): CD27 ligand, CD70 antigen, Cd27l, Cd27lg, Tnfsf7, Tumor necrosis factor ligand superfamily member 7
• UNSPSC Code: 12352203

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: FR70, monoclonal
• mol wt: calculated mol wt 21.92 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG2bκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_035747
• UniProt accession no.: O55237
• storage temp.: -10 to -25°C
• Gene Information: mouse ... Cd70(21948)

Description

Specificity

Clone FR70 is a rat monoclonal antibody that detects mouse CD70. It targets an epitope within the extracellular domain.

Immunogen

A20 cells

Application

Quality Control Testing

Evaluated by Western Blotting in mouse splenocytes lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CD70 in mouse splenocytes lysate.

Tested Applications

Inhibition Assay: A representative lot inhibited binding of a murine CD27 Fc fusion protein to mouse CD70 in Inhibition Assays (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected CD70 in P815 cells overexpressing mouse CD70.

Flow Cytometry Analysis: A representative lot detected CD70 in Flow Cytometry application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Western Blotting Analysis: A representative lot detected CD70 in Western Blotting application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD70 in Immunoprecipitation application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

CD70 antigen (UniProt: O55237; also known as CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7) is encoded by the Cd70 (also known as Cd27l, Cd27lg, Tnfsf7) gene (Gene ID: 21948) in mouse. CD70 is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) family. The extracellular domain is involved in binding to its receptor, CD27, which is a member of the TNF receptor family. CD70, together with CD27, plays a critical role in the immune system and involved in T and B cell activation and differentiation and in the formation and maintenance of memory T cells, which are essential for long-term immunity. CD70 expression is tightly regulated and primarily found on activated lymphocytes and dendritic cells. Overexpression of CD70 in tumors can lead to immune evasion by inducing T cell exhaustion and apoptosis through chronic CD70-CD27 signaling. Targeting CD70 or CD27 is being explored as a therapeutic strategy in oncology. Dysregulated CD70 expression can contribute to autoimmune conditions and chronic infections as well. (Ref.: Flieswasser, T., et al., 2022, J. Exp. Clin. Cancer Res., 41:12, Oshima, H., et al. (1998). Int Immunol. 10(4):517-26)

Physical form

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable