MABF3145
Anti-Envelope glycoprotein B Antibody, HCMV Antibody, clone 27-156
Synonym(s):
gB
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
27-156, monoclonal
mol wt
calculated mol wt 101.95 kDa
observed mol wt ~120-140 kDa
purified by
using protein G
species reactivity
virus
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
isotype
IgG2bκ
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
storage temp.
-10 to -25°C
Gene Information
vaccinia virus ... HA(3654615)
General description
Envelope glycoprotein B (UniProt: P13201; also known as gB) is encoded by the gB (also known as UL55) gene (Gene ID: 3077424) in human cytomegalovirus (HHV-5). Human cytomegalovirus (HCMV), a member of the β-Herpesviridae family, is a major cause of morbidity and mortality in organ transplant recipients and patients with AIDS. Its envelope glycoprotein B is reported to be essential for the infectivity and is considered to be one of the most immunogenic virus-encoded proteins. It facilitates the entry of HCMV entry into cells by binding receptors and mediating membrane fusion. Glycoprotein B is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains a virion surface domain (aa 23-751), a transmembrane domain (aa 752-772), and an intra-virion domain (aa 773-907). The amino acid sequences 152-158 and 237-244 are critical for fusion and/or binding to host cell membrane. During virion morphogenesis, envelope glycoprotein B probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is then transported to the cell surface from where it is endocytosed and is directed to the trans-Golgi network. The ectodomain of HCMV glycoprotein B forms rosette-like aggregates that are similar to rosettes formed by the EBV glycoprotein B ectodomain. Four exposed hydrophobic residues in its fusion loop are shown to be essential for rosette formation. A linear domain of glycoprotein B, designated at AD-1, represents a dominant antibody binding site on this protein and is also required for oligomerization of glycoprotein B. (Ref.: Sharma, S., et al. (2013). Virology. 435(2); 239-249; Britt, WJ., et al. (2005). J. Virol. 79(7); 4066-4079; Jean, F., et al. (2000). Proc. Natl. Acad. Sci. USA. 97(6); 2864-2869; Speckner, ., et al. (1999). J. Gen. Virol. 80(8); 2183-2191; Schoppel, K., et al. (1996). Virology. 216(1); 133-145).
Specificity
Clone 27-156 is a mouse monoclonal antibody that detects the envelope glycoprotein B in Human cytomegalovirus (HCMV). It targets an epitope within the antigenic domain 1 (AD1) within the C-terminal half.
Immunogen
Whole Human cytomegalovirus (HCMV).
Application
Quality Control Applications
Evaluated by Western Blotting with recombinant HCMV glycoprotein B.
Western Blotting Analysis (WB): A 1:200,000 dilution of this antibody detected recombinant HCMV glycoprotein B.
Tested applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated HCMV Envelope glycoprotein B in Immunoprecipitation applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Vanarsdall, A.L., et al. (2008). J Virol. 82(23);11837-50).
Immunocytochemistry Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunocytochemistry applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); :2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64).
Neutralizing: A representative lot neutralized Envelope glycoprotein B of HCMV. (Schoppel, K., et al. (1996). Virology. 216(1);133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8); 1999-2007; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
Immunofluorescence Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunofluorescence applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1);113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
ELISA Analysis: A representative lot detected HCMV Envelope glycoprotein B in ELISA applications (Sharma, S., et al. (2013). Virology. 435(2); 239-49).
Western Blotting Analysis: A representative lot detected HCMV Envelope glycoprotein B in Western Blotting applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Schoppel, K., et al. (1996). Virology. 216(1); 133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8);1999-2007; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Sharma, S., et al. (2013). Virology. 435(2); 239-49; Khanh Le-Trilling, V. T., et al. (2020). Cell Rep. 30(7); 2248-2260).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant HCMV glycoprotein B.
Western Blotting Analysis (WB): A 1:200,000 dilution of this antibody detected recombinant HCMV glycoprotein B.
Tested applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated HCMV Envelope glycoprotein B in Immunoprecipitation applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Vanarsdall, A.L., et al. (2008). J Virol. 82(23);11837-50).
Immunocytochemistry Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunocytochemistry applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); :2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64).
Neutralizing: A representative lot neutralized Envelope glycoprotein B of HCMV. (Schoppel, K., et al. (1996). Virology. 216(1);133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8); 1999-2007; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
Immunofluorescence Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunofluorescence applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1);113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
ELISA Analysis: A representative lot detected HCMV Envelope glycoprotein B in ELISA applications (Sharma, S., et al. (2013). Virology. 435(2); 239-49).
Western Blotting Analysis: A representative lot detected HCMV Envelope glycoprotein B in Western Blotting applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Schoppel, K., et al. (1996). Virology. 216(1); 133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8);1999-2007; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Sharma, S., et al. (2013). Virology. 435(2); 239-49; Khanh Le-Trilling, V. T., et al. (2020). Cell Rep. 30(7); 2248-2260).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Physical form
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MABF3145-25UG:
MABF3145-100UG:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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