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Safety Information

MABE972

Sigma-Aldrich

Anti-PRDM5 Antibody

clone 57-20, from mouse

Synonym(s):

PRDM5, PR domain zinc finger protein 5, PR domain-containing protein 5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

57-20, monoclonal

species reactivity

mouse

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_061169

UniProt accession no.

Q9NQX1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Prdm5(70779)

General description

PR domain zinc finger protein 5 (UniProt Q9NQX1) is encoded by the PRDM1 gene (also known as BCS2, PFM2) in human (Gene ID 11107). PRDM proteins constitute a family of transcriptional regulators that are characterized by the presence of variable numbers of zinc finger domains and an N-terminal PR domain. PRDM5 can negatively regulate gene transcriptions by recruiting the G9a histone methyltransferase and histone deacetylases. PRDM5 can promote transcription by targeting enhancer-like elements or recruiting RNA polymerase II binding. PRDM5 is highly expressed in murine embryonic stem cells (mESCs) and associates with complexes involved in chromatin organization, such as Ctcf, Smc1 (cohesin), and TFIIIC, which indicates their involvement in chromatin organization. PRDM5 is often found silenced in human breast, ovarian, liver, and cervical cancers.

Specificity

Expected to react with all four spliced isoforms

Immunogen

GST-tagged recombinant protein corresponding to human PRDM5 near the N-terminus.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated PRDM5 in mouse embryonic fibroblasts (Data courtesy of Dr. Anders H. Lund, University of Copenhagen, Denmark).
Immunoprecipitation: A representative lot of this antibody immunoprecipitated PRDMS E9 and E10 intrahepatic cholangiocarcinoma (ICC) cells. (Seehawer, M., et al. (2018). Nature 562(7725); 69-75).
This Anti-PRDM5 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of PRDM5 .

Quality

Evaluated by Western Blotting in mouse ESC lysate.

Western Blotting Analysis: 8.0 µg/mL of this antibody detected PRDM5 in 10 µg of mouse ESC lysate.

Target description

~73 kDa observed. 73.1 kDa (isoform 1, calculated), 69.9 kDa (isoform 2, calculated), 12.9 kDa (isoform 3, calculated), 58.1 kDa (isoform 4, calculated).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABE972:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marco Seehawer et al.
Nature, 562(7725), 69-75 (2018-09-14)
Primary liver cancer represents a major health problem. It comprises hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC), which differ markedly with regards to their morphology, metastatic potential and responses to therapy. However, the regulatory molecules and tissue context that commit
Giorgio Giacomo Galli et al.
Molecular and cellular biology, 33(22), 4504-4516 (2013-09-18)
PRDM proteins belong to the SET domain protein family, which is involved in the regulation of gene expression. Although few PRDM members possess histone methyltransferase activity, the molecular mechanisms by which the other members exert transcriptional regulation remain to be

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