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MABE453

Sigma-Aldrich

Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4

clone 7B4, from mouse

Synonym(s):

H2BUb1, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l, H2BUbK119, H2BK119Ub

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7B4, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_003509

UniProt accession no.

P62807

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HIST1H2BC(8347)

General description

H2BUb1, also known as Histone H2B.1 A, or Histone H2B.a (H2B/a), or Histone H2B.g (H2B/g), or Histone H2B.h (H2B/h), or Histone H2B.k (H2B/k) or Histone H2B.i (H2B/I;H2B/I), and encoded by the gene HIST1H2BC or HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI/H2BFL, H2BFH, H2BFG, H2BFA, H2BFK, is a core component of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2BUb1 also has broad antibacterial activity and may contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. H2BUb1 is localized to the nucleus and chromosomes in healthy cells.

Immunogen

Ovalbumin-conjugated corresponding to human monoubiquityl Histone H2B (Lys119).

Application

Detect Histone H2B using this mouse monoclonal antibody, Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4 validated for use in western blotting & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in hormone deprived MCF7 cell lysates, which were pretreated with vehicle (ethanol) or Bortezomid before incubating with 17Beta-Estradiol (Prenzel, T., et al. (2011). AACR. OF1-OF15).
Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in differentiated hMSCs that were truncated with control and demostrated a loss of signal in differntiated hMSCs that ere transfected with RNF50 siRNA (Karpiuk, O., et al. (2012). Molecular Cell. 46:705-713).
Immunohistochemistry Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in malignant, metastatic breast tumor and normal adjacent tissues (Prenzel, T., et al. (2011). AACR. OF1-OF15).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected monoubiquityl Histone H2B (Lys119) in HeLa cell lysates treated with and without Nocodazole.

Target description

~20 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABE453:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The histone H2B monoubiquitination regulatory pathway is required for differentiation of multipotent stem cells.
Karpiuk, Oleksandra, et al.
Molecular Cell, 46, 705-713 (2012)
Mei-Jun Zhao et al.
Oncotarget, 7(8), 8956-8967 (2016-01-21)
The ubiquitin ligase RNF8 promotes the DNA damage response (DDR). We observed that the expression of RNF8 was increased in bladder cancer cells and that this change in RNF8 expression could be reversed by adenovirus-mediated shRNA treatment. Moreover, we found

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