MABC1144

Anti-LMO-2 (Rhombotin-2) Antibody, clone 1A9-3B11

clone 1A9-3B11, from mouse

• Synonym(s): Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1A9-3B11, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin)
• isotype: IgG1κ
• NCBI accession no.: NP_005565.2
• UniProt accession no.: P25791
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... LMO2(4005)

Description

General description

Rhombotin-2 (UniProt P25791; also known as Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2) is encoded by the LMO2 (also known as RBTN2, RBTNL1, RHOM2, TTG2) gene (Gene ID 4005) in human. Rhombotin-2 is 158 amino acids, small cysteine-rich protein with multiple functions. A large portion of its structure is occupied by tandemly repeated cysteine-rich zinc-finger binding motifs, the LIM domains. However, it does not have any identifiable DNA-binding motifs. It is found predominantly in the nucleus or in the cytoplasm depending upon both the tissue and the cell type. For example, in kidney it is predominantly localized in the cytoplasm, but in hematopoietic cells it has nuclear localization. LMO2 is reported to be a proto-oncogene only in the context of T lymphocytes and plays a role in T-cell leukemogenesis. It also has distinct functions in erythropoiesis. It acts with TAL1/SCL to regulate red blood cell development and interacts with LDB1 to maintain erythroid precursors in an immature state. It is reported to interact with retinoblastoma-binding protein 2 (RBP2) and human Bex2 (brain expressed X-linked), both of which are shown to enhance its transcriptional activity. A chromosomal aberration involving LMO2 has been linked to T-cell acute lymphoblastic leukemia (T-ALL).
Ref.: Mao S., et al. (1997) Oncogene 14, 1531-1539.

Immunogen

GST-tagged recombinant human LMO-2 (Rhombotin-2).

Application

Detect LMO2 using this mouse monoclonal Anti-LMO-2 (Rhombotin-2) Antibody, clone 1A9-3B11, Cat. No. MABC1144, validated for use in Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LMO-2 (Rhombotin-2) in human lymphoma and cerebellum tissue sections.

Clone 1A9-3B11 exhibits similar property as clone 1A9-1 subcloned from the same parental hybridoma culture (Natkunam, Y., et al. (2007). Blood. 109(4):1636-1642). Please refer to Natkunam, Y., et al. (2007) regarding Immunohistochemistry and Western Blotting applications using the related subclone 1A9-1.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry in human kidney tissue sections.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LMO-2 (Rhombotin-2) in human kidney tissue section.

Target description

18.36/25.04 kDa (human isoform 1/3) calculated. ~24 kDa reported (Natkunam, Y., et al. (2007). Blood. 109(4):1636-1642).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1