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MAB8560

Sigma-Aldrich

Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4

ascites fluid, clone 583-G8-G2-A4, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

300

antibody form

ascites fluid

antibody product type

primary antibodies

clone

583-G8-G2-A4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

isotype

IgG1

shipped in

wet ice

Specificity

Reacts with the Poliovirus 1.

Application

Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF.
IFA at 1:100-1:400.

Optimal working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air Dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3

6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Storage and Stability

Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB8560:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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