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MAB810-M

Sigma-Aldrich

Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

antibody form

purified antibody

Quality Level

300

clone

8B1.2
monoclonal

purified by

using Protein A

species reactivity

human, mouse

concentration

(Please refer to the Certificate of Analysis for the lot-specific concentration.)

technique(s)

immunofluorescence: suitable

isotype

IgG2a

General description

Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Specificity

Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.

Application

ELISA 1:800

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.

Works on paraffin embedded tissue sections.

FACS: See online protocol.

Final working dilutions and protocols must be determined by end user.

Physical form

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Other Notes

Cytomegalovirus Control Slides, Catalogue Number 5027-5

Disclaimer

This product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB810-KC:
MAB810-K:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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