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Safety Information

MAB5606Z

Sigma-Aldrich

Anti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free)

clone GD1a-1, from mouse

Synonym(s):

GD1a Ganglioside

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GD1a-1, monoclonal

species reactivity

bovine, human, fish, mouse, rat

species reactivity (predicted by homology)

vertebrates (based on 100% sequence homology)

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

shipped in

dry ice

target post-translational modification

unmodified

General description

Gangliosides are sialic acid-containing oligoglycosylceramides. The German scientist Ernst Klenk used the term ganglioside in 1942 to refer to lipids isolated from ganglions. Gangliosides are grouped based on the number of sialic acid residues per molecule, GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, GQ contain four sialic acid residues. Glycolipids constitute >80% of mammalian brain glycans, with gangliosides being the major glycans in nerve cells. Among the hundreds of ganglioside structures, GM1a, GD1a, GD1b and GT1b comprise up to 97% of gangliosides in the human brain. These four gangliosides share the same neutral tetrasaccharide core (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1′Cer), but differ from each other by their sialic acid residue(s). GM1a and GD1b contain one or two sialic acids on the internal galactose, respectively, and no sialic acid on the terminal galactose. The St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III sialyltransferase, respectively) are largely responsible for ganglioside terminal α2-3 sialylation of GM1a and GD1b to produce GD1a and GT1b in the brain. Brain GD1a and GT1b in St3gal2-null mice is about half the level in wild-type mice, while GD1a and GT1b level in St3gal2/3-double-null mice is less than 5% of the level in wild-type mice. St3gal2/3-double-null mice are half the weight of wild-type mice at weaning and display early hindlimb dysreflexia.

Specificity

GD1a ganglioside. The antibody shows approximately 10% reactivity to GalNAc-GD1a and GT1aα. Preferentially binds to motor myelinated fibers in cross-sections of ventral roots and sciatic nerves.

Immunogen

KLH-conjugated GD1a ganglioside.

Application

Anti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free) is an antibody against GD1a Ganglioside for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GD1a Ganglioside in human prostate tissue.
ELISA Analysis: Target specificity of a representative lot was determined by ELISA analysis (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
Immunohistochemistry Analysis: A representative lot detected GD1a immunoreactivity in fresh-frozenmid-sagittal brain sections from wild-type and St3gal2/3-double-null mice (Sturgill, E.R., et al. (2012). Glycobiology.22(10):1289-1301).
Immunohistochemistry Analysis: A representative lot detected GD1a immunoreactivity in various fish brain regions using free-floating sections (Viljetić, B., et al. (2012). Biochim Biophys Acta. 1820(9):1437-1443).
Immunohistochemistry Analysis: A representative lot detected GD1a in fresh-frozen human and rat brain sections by both fluorescent and non-fluorescent immunohistochemistry (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
Immuno-overlay Assay: A representative lot detected bovine brain GD1a, as well as GD1a in human ventral and dorsal root gangliosides extracts (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GD1a Ganglioside in human heart tissue.

Linkage

Replaces: MAB5606

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with no azide

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB5606Z:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biosynthesis of the major brain gangliosides GD1a and GT1b.
Sturgill, ER; Aoki, K; Lopez, PH; Colacurcio, D; Vajn, K; Lorenzini, I; Majic, S; Yang et al.
Glycobiology null
Karin Säljö et al.
Glycobiology, 27(4), 291-305 (2016-12-10)
High expectations are held for human-induced pluripotent stem cells (hiPSC) since they are established from autologous tissues thus overcoming the risk of allogeneic immune rejection when used in regenerative medicine. However, little is known regarding the cell-surface carbohydrate antigen profile
Localization of major gangliosides in the PNS: implications for immune neuropathies.
Gong, Y; Tagawa, Y; Lunn, MP; Laroy, W; Heffer-Lauc, M; Li, CY; Griffin, JW; Schnaar, RL; Sheikh, KA
Brain null
Barbara Viljetić et al.
Biochimica et biophysica acta, 1820(9), 1437-1443 (2011-12-31)
Mono-, di- and trisialo gangliosides are major glycosphingolipids in the brain of higher vertebrates involved in lipid raft assembly. In contrast, the fish brain is abundant in polisialo-gangliosides, whose function is implicated in the modulation of repulsive and attractive intercellular

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