MAB5234

Anti-Neurocan Antibody

Chemicon^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: chicken
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_004386.2
• UniProt accession no.: O14594
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NCAN(1463)

Description

General description

Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.

Specificity

Neurocan, chicken. Recognizes the middle region of neurocan.

Application

Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.

Western blot: <0.5 μg/mL

Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.

Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

Immunoprecipitation: 1 μg/mL

ELISA: 1 μg/mL, excellent for core protein, good for monomer

Immunocytochemistry: not tested

Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable