MAB3236

Anti-Cytokeratin 18 Antibody, clone RCK106

clone RCK106, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RCK106, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000224.2; NM_199187.1
• UniProt accession no.: P05783
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... KRT18(3875)

Description

General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Specificity

Cytokeratin 18. MAB3236 reacts exclusively with human glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells as well as adenocarcinomas originating from them.

SPECIES REACTIVITY:

Human only. Does not react with other species.

Immunogen

Cytoskeletal preparation of human bladder carcinoma cell line T24.

Application

Anti-Cytokeratin 18 Antibody, clone RCK106 detects level of Cytokeratin 18 & has been published & validated for use in FC, WB, IC, IH(P).

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Western blot

Immunohistochemistry on frozen and paraffin embedded tissue sections.

Immunocytochemistry

Flow cytometry

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: 04-586

Physical form

Format: Purified

Liquid in buffer with 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable