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Safety Information

MAB3043

Sigma-Aldrich

Anti-Connexin 35/36 Antibody, clone 9D7.2

clone 9D7.2, Chemicon®, from mouse

Synonym(s):

Cx35/36

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9D7.2, monoclonal

species reactivity

rabbit, fish

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

Q8JFD6

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rabbit ... Gjd2(100341036)

Specificity

Recognizes Perch Connexin 35. Also recognizes rabbit Connexin 36, the gene homologue of Connexin 35. Labels gap junctions in retinas of perch and rabbit in immunohistochemistry.

Immunogen

Recombinant Perch Connexin 35

Application

Anti-Connexin 35/36 Antibody, clone 9D7.2 detects level of Connexin 35/36 & has been published & validated for use in WB, IH.
Immunohistochemistry

Western blot: 1:500-1:1000

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.1M Citrate buffer with 50% glycerol and 0.1% Sodium Azide as a preservative.

Storage and Stability

Maintain at -20°C for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB3043:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Trafficking of gap junction channels at a vertebrate electrical synapse in vivo.
Flores, CE; Nannapaneni, S; Davidson, KG; Yasumura, T; Bennett, MV; Rash, JE; Pereda, AE
Proceedings of the National Academy of Sciences of the USA null
John E Rash et al.
Neuron, 79(5), 957-969 (2013-09-10)
Electrical synapses are abundant in the vertebrate brain, but their functional and molecular complexities are still poorly understood. We report here that electrical synapses between auditory afferents and goldfish Mauthner cells are constructed by apposition of hemichannels formed by two
Cong Yao et al.
Journal of neurophysiology, 112(9), 2102-2113 (2014-08-01)
In contrast to the knowledge of chemical synapses, little is known regarding the properties of gap junction-mediated electrical synapses in developing zebrafish, which provide a valuable model to study neural function at the systems level. Identifiable "mixed" (electrical and chemical)
J E Rash et al.
Neuroscience, 285, 166-193 (2014-12-03)
Gap junctions provide for direct intercellular electrical and metabolic coupling. The abundance of gap junctions at "large myelinated club ending (LMCE)" synapses on Mauthner cells (M-cells) of the teleost brain provided a convenient model to correlate anatomical and physiological properties
Shu Xie et al.
Cell reports, 37(3), 109853-109853 (2021-10-24)
Currently, many genetic methods are available for mapping chemical connectivity, but analogous methods for electrical synapses are lacking. Here, we present pupylation-based interaction labeling (PUPIL), a genetically encoded system for noninvasively mapping and stamping transient electrical synapses in the mouse
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