MAB2630

Anti-KRIT-1 Antibody, clone 15

clone 15B2, from mouse

• Synonym(s): Cerebral cavernous malformations 1 protein, Krev interaction trapped 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: 15B2, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_919436.1
• UniProt accession no.: O00522
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KRIT1(889)

Description

General description

Cerebral cavernous malformation (CCM), a disease associated with defective endothelial junctions, results from autosomal dominant CCM1 mutations that cause a loss of KRIT-1 protein function. CCM1 has been identified as the KRIT-1 gene. The protein product, KRIT-1, also known as Krev interaction trapped protein 1, is a microtubule-associated protein involved in endothelial cell-cell and cell-extracellular matrix interactions. KRIT-1 binds to Rap1 that maintains the integrity of endothelial junctions. It is reported that KRIT-1 colocalizes and is physically associated with junctional proteins via its band 4.1/ezrin/radixin/moesin (FERM) domain, (Glading, 2007).

Specificity

The antibody recognizes KRIT-1.

Immunogen

Epitope: FERM domain

GST-tagged recombinant protein corresponding to human KRIT-1 at and around the FERM domain.

Application

Anti-KRIT-1 Antibody, clone 15 detects level of KRIT-1 & has been published & validated for use in IC, IP & WB.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected KRIT-1 in HeLa, A431, and HUVECs.

Target description

~ 84 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa, A431, and HUVECs.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable