MAB1598

Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8

clone 7E3-1B8, Chemicon^®, from mouse

• Synonym(s): PSD-95
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7E3-1B8, monoclonal
• species reactivity: bovine, rat, mouse
• species reactivity (predicted by homology): human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P78352
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Dlg4(100137840) ; human ... DLG4(1742) ; mouse ... Dlg4(13385) ; rat ... Dlg4(29495)

Description

General description

The postsynaptic density (PSD) is an electron dense structure just beneath the postsynaptic membrane. Several functions have been proposed for the PSD including regulating receptor number and clustering, anchoring signal transduction molecules at the synapse and mediating adhesion between the presynaptic and postsynaptic membranes. The most abundant PSD protein is the [alpha] subunit of the type II calcium/calmodulin dependent protein kinase ([alpha]CaMKII). This protein is likely to play a role in the calcium-mediated signal transduction at the synapse that mediates certain forms of synaptic plasticity. Another major PSD protein is PSD-95, a member of the guanylate kinase family (GUK) of proteins.

Specificity

Reacts with recombinant rat PSD-95 and identifies a band at approximately ~100 kDa, corresponding to PSD-95, on western blots of rat brain, mouse brain and bovine brain extracts. Also present in rat brain and mouse brain tissue extracts are bands of approximately 80 kDa and 50 kDa. PSD-95 is believed to initiate NMDA receptor clustering on the post synaptic membrane.

Immunogen

Recombinant rat PSD-95

Application

Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8 is an antibody against Post Synaptic Density Protein 95 for use in IC, IP & WB.

Immunocytochemistry:
A previous lot of this antibody was used in IC.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by the end user.

Quality

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected PSD-95 on 10 µg of Mouse brain lysates.

Target description

~ 95 kDa

Linkage

Replaces: 04-1066

Physical form

Format: Purified

Purified mouse monoclonal IgG1 liquid in buffer containing PBS, pH 7.2, with 0.09% sodium azide and 50% glycerol.

Analysis Note

Control
Mouse brain, rat brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2