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Safety Information

MAB1379

Sigma-Aldrich

Anti-CD54 Antibody, extracellular, clone 84H10

clone 84H10, Chemicon®, from mouse

Synonym(s):

ICAM-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

84H10, monoclonal

species reactivity

human

should not react with

canine

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_000201.1

UniProt accession no.

P05362

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ICAM1(3383)

General description

This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells.

Specificity

Recognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa.

Immunogen

Epitope: extracellular
K562 cell line

Application

Detect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH.
Flow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells.

Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.

Immunoprecipitation: 5 μg/ 10e7 cells

Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Physical form

Affinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide.
Format: Purified

Storage and Stability

Store at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20ºC. Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1379:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Li Wang et al.
Haematologica, 96(6), 927-931 (2011-02-19)
Vascular endothelial growth factor-A, an angiogenesis stimulator expressed on both tumor endothelial and malignant T cells, is involved in tumor progression in T-leukemia/lymphoma. Here, we assessed the impact of therapeutic vascular endothelial growth factor-A blockade on tumor-endothelial cell interaction and
Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin.
Burns, MP; DePaola, N
American Journal of Physiology. Heart and Circulatory Physiology null
Timo Rath et al.
Scandinavian journal of gastroenterology, 45(10), 1186-1196 (2010-06-24)
Matrix metalloproteinases (MMPs) are considered the predominant proteases in the pathogenesis of mucosal ulcerations associated with inflammatory bowel disease (IBD). Whether the malignancy associated MMP-7 and MMP-13 or the recently cloned MMP-28 convey a certain meaning for intestinal homeostasis and
Synergistic induction of intercellular adhesion molecule-1 by the human cytomegalovirus transactivators IE2p86 and pp71 is mediated via an Sp1-binding site.
Martina Kronschnabl, Thomas Stamminger
The Journal of General Virology null

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