MAB1072

Anti-Erythropoietin Antibody, clone 1C8

clone 1C8, Chemicon^®, from mouse

• Synonym(s): erythropoietin, Epoetin, epoetin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• clone: 1C8, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NM_000799.2
• UniProt accession no.: P01588
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

Specificity

Reacts with human Erythropoietin (Epo).

Immunogen

Recombinant human Epo.

Application

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Metabolic Hormones & Receptors

Quality

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

Target description

34 kDa (glycosylated)

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable