HTS109RTA

Ready-to-Assay 5-HT _2B Serotonin Receptor Frozen Cells

Human

• UNSPSC Code: 41106514
• eCl@ss: 32011203
• NACRES: NA.76
• biological source: human
• manufacturer/tradename: Ready-to-Assay

Properties

• Product Name: Ready-to-Assay 5-HT _2B Serotonin Receptor Frozen Cells,
• biological source: human
• Quality Level: 100
• manufacturer/tradename: Ready-to-Assay
• technique(s): calcium flux assay: suitable
• NCBI accession no.: NM_000867
• detection method: fluorometric

Description

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). The 5-HT2B receptor, which couples to Gq/11 to increase intracellular calcium, is expressed in embryonic and adult cardiovascular tissues, gut and brain from the rat, mouse, and human species. A role for 5-HT2B receptors was suggested in the cardiopathy associated with fenfluramine (Fitzgerald et al., 2000) Millipore′s cloned human 5-HT2B expressing cell line is made in the CHO host, which supports high levels of recombinant 5-HT2B expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at 5-HT2B.

Cell Line Description

• GPCR Cell Lines
• Host cells: CHO (Chinese hamster ovary cells)

Application

Human 5-HT2B GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

• GPCR Class: A
• Protein Target: 5-HT2B
• Target Sub-Family: Serotonin

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer

This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable