HMYOMAG-56K
MILLIPLEX® Human Myokine Magnetic Bead Panel
The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.
About This Item
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 100%
(Apelin)
accuracy: 92%
(IL-15)
accuracy: 96%
(FSTL-1)
accuracy: 97%
(LIF)
accuracy: 98%
(Oncostatin M (OSM))
sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)
sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)
sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)
sensitivity: 4 pg/mL
(MinDC+2SD; LIF)
sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)
sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)
sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)
sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))
sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)
sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))
sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))
sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))
sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)
sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)
standard curve range: 0.6-2,400 pg/mL
(IL-6)
standard curve range: 10-40,000 pg/mL
(LIF)
standard curve range: 122-500,000 pg/mL
(Apelin)
standard curve range: 2-10,000 pg/mL
(BDNF)
standard curve range: 2-10,000 pg/mL
(IL-15)
standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))
standard curve range: 24-100,000 pg/mL
(FABP3)
standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))
standard curve range: 244-1,000,000 pg/mL
(Irisin)
standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))
standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)
standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)
standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)
standard curve range: 5-20,000 pg/mL
(FGF-21)
standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))
inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)
inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)
inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))
inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)
inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)
inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))
inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)
inter-assay cv: <15%
intra-assay cv: <10%
(LIF)
inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)
inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))
inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)
inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
MILLIPLEX® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Application
- Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
- Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
- NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
- Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
- Research Category: Bone
- Research Subcategory: Metabolism
Features and Benefits
Other Notes
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
HMYOMAG-56K-10:
HMYOMAG-56K-12:
HMYOMAG-56K-15:
HMYOMAG-56K-09:
HMYOMAG-56K-13:
HMYOMAG-56K-01:
HMYOMAG-56K-05:
HMYOMAG-56K-14:
HMYOMAG-56K-04:
HMYOMAG-56K-07:
HMYOMAG-56K-06:
HMYOMAG-56K:
HMYOMAG-56K-03:
HMYOMAG-56K-11:
HMYOMAG-56K-02:
HMYOMAG-56K-08:
Certificates of Analysis (COA)
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