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Key Documents

Safety Information

HC08

Millipore

Fluorescent Mounting Media, Aqueous

This Fluorescent Mounting Media, Aqueous is validated for use in Immunofluorescence for the detection of Fluorescent Mounting Media Aqueous.

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.47

form

liquid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−20°C

General description

An aqueous mounting medium designed to preserve and enhance the labeling of immunoreactivity in tissue sections. Sustains the fluorescent signal from labeled samples on slides. Inclusion of 4,6-diamidino-2-phenylindole (DAPI; Cat. No. 268298) allows for the visualization of unlabeled (non-apoptotic) cells. Suitable for use with a wide variety of fluorophores.
Aqueous mounting media designed to preserve and enhance the labeling of tissue sections or cell preparations fixed on slides. Suitable for many fluorophores including fluorescein, Oregon Green, Alexa 488, rhodamine, Texas Red, Cyanine (Cy) 3, and Alexa 546. Inclusion of DAPI in the Fluorescent Mounting Media allows for the visualization of all cells at an excitation wavelength of 330-380 nm.

Application

Immunofluorescence (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HC08-8ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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