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Safety Information

FCMAB230P

Sigma-Aldrich

Milli-Mark® Mouse IgG1-k, clone MOPC-21, PE conjugate

This Milli-Mark Anti-mouse IgG1-PE Antibody, clone MOPC-21 is validated for use in FC for the detection of mouse IgG1.

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

PE

antibody form

purified immunoglobulin

clone

MOPC-21, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

General description

The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Specificity

This mouse IgG1 isotype control antibody is not reactive with any known antigen.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Isotype Control Antibodies

Quality

Evaluated for use as flow cytometry isotype control using Jurkat Cells.

Physical form

Immunoelectrophoresis
Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

FCMAB230P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Robin F Chan et al.
Nucleic acids research, 45(11), e97-e97 (2017-03-24)
Methylome-wide association studies are typically performed using microarray technologies that only assay a very small fraction of the CG methylome and entirely miss two forms of methylation that are common in brain and likely of particular relevance for neuroscience and

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