CGTMAG-98K

MILLIPLEX^® Canine Gut Hormone Magnetic Bead Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: Amylin (total), Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, Insulin, Leptin, Pancreatic Polypeptide (PP), PYY (total).

• Synonym(s): canine gut endocrine metabolic hormone immunoassay panel, luminex canine gut metabolic endocrine hormone multiplex assay, millipore canine gut metabolism hormone multiplex kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: canine
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 100%; (GLP-1(Active)); accuracy: 101%; (Glucagon); accuracy: 103%; (Amylin(Total)); accuracy: 98%; (PYY(total)); accuracy: 99%; (GIP); accuracy: 99%; (Ghrelin(Active)); accuracy: 99%; (Leptin); accuracy: 99%; (PP); sensitivity: 0.6 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); GIP (total)); sensitivity: 13.1 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); PYY (Total)); sensitivity: 3.3 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); Ghrelin (active)); sensitivity: 3.6 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); PP); sensitivity: 43.6 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); Glucagon); sensitivity: 5.9 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); Amylin (total)); sensitivity: 79.3 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); Insulin); sensitivity: 9.0 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); GLP-1 (active)); sensitivity: 92.1 pg/mL; (Minimum Detectable Concentration (MinDC +2SD); Leptin); standard curve range: 0.5-2,000 pg/mL; (GIP); standard curve range: 0.7-3,000 pg/mL; (Ghrelin (Active)); standard curve range: 2.4-10,000 pg/mL; (GLP-1 (Active), PP, PYY (Total)); standard curve range: 24.4-100,000 pg/mL; (Insulin, Leptin); standard curve range: 4.9-20,000 pg/mL; (Glucagon)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Gut hormones are a group of polypeptides secreted from the gastrointestinal tract. These peptide hormones play very important roles in the regulation of gut motility and secretion, pancreatic islet hormone secretion, appetite, and energy expenditure.

The MILLIPLEX^® Canine Gut Hormone Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine plasma, serum or tissue supernatants or tissue extraction samples: Amylin (total), Active Ghrelin, GIP, GLP-1, Glucagon, Insulin, Leptin, PP and PYY. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
No significant cross-reactivity with other hormones tested.

Application

• Analytes: Amylin* (Total), Ghrelin* (Active), GIP (Total), GLP-1* (Active), Glucagon, Insulin, Leptin, Pancreatic Polypeptide (PP) and PYY (total)
• Recommended Sample Type: Canine serum, plasma, tissue supernatants or tissue extraction samples; (*) specific analytes require protease inhibitor(s) during sample collection
• Recommended Sample Dilution:
• 50 μL per well of undiluted serum or plasma
• Assay Run Time: Overnight (16-18 hr) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Obesity, Diabetes

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Canine Gut Hormone Standard, 1 vial, lyophilized

Canine Gut Hormone Control 1, 1 vial, lyophilized

Canine Gut Hormone Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 bottle, 1 mL

Serum Matrix, 1 bottle, lyophilized

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Canine Gut Hormone Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H411
• Precautionary Statements: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects