CC1048

MMP-9, human, proform

• Synonym(s): Progelatinase B, 92 kDa Type IV Collagenase
• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Assay: >95% (total protein)
• form: liquid
• specific activity: >1200 mU/mg (specific activity of activated MMP-9 after trypsin activation)
• manufacturer/tradename: Chemicon^®
• concentration: 0.2 mg/mL
• NCBI accession no.: NM_004994.2
• UniProt accession no.: P14780
• shipped in: dry ice
• Gene Information: human ... MMP9(4318)

Description

General description

When measured with the peptide substrate dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg [Masui et al., 1977] the specific activity of MMP-9 monomer

Application

ACTIVATION:

An aliquot of 10 μL MMP-9 monomer is mixed with 20 μL trypsin solution (see below) and activation buffer in a total volume of 100 μL. The mixture is incubated for 20 min at 37°C. Thereafter trypsin is inhibited by addition of 10 μL aprotinin solution.

Trypsin solution: 0.50 mg TPCK-trypsin/mL activation buffer. The solution is stored in aliquots at -20°C.

Activation Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2.

Aprotinin solution: 1 mg aprotinin/mL activation buffer. The solution is stored at -20°C.

Unit Definition

Specific Activity: where 1 U is the activity that hydrolyzes 1 mmol peptide (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 minute under the assay conditions described by Knight ,et al

Physical form

Provided as a liquid in 50 mM Tris-HCl, pH 7.0, 200 mM NaCl, 5 mM CaCl2, ,1 μM ZnCl2, 0.05% Brij-35, 0.05% NaN3.

Storage and Stability

Maintain frozen at -70°C in undiluted aliquots. The enzyme may be stored at -20°C for several without significant loss of activity. Repeated freezing and thawing should be avoided.

Analysis Note

MMP-9 monomer appears as a major band at 92 kDa in non-reducing SDS-PAGE

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable