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Key Documents

Safety Information

CC102A

Sigma-Aldrich

Human Integrin αVβ3, Octyl-β-D-glucopyranoside Formulation

Synonym(s):

Vitronectin Receptor, CD51/CD61

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100

Assay

>95% (silver stain, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

0.25 mg/mL

NCBI accession no.

NM_002210.2

UniProt accession no.

P06756

Gene Information

human ... ITGAV(3685)

General description

Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Product was tested and found negative for HIV and Hepatitis B.

Application

Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 .

Physical form

Purified protein inTris buffered saline with 100 mM Octyl-beta-D-glucopyranoside, and sodium azide. This format of alphaVbeta3 is appropriate for functional evaluations.

Storage and Stability

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/ thaw cycles.

Analysis Note

Specific Activity: Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaVbeta3 integrin will also interact with other alphaVbeta3 binding proteins in an RGD-dependent fashion.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

CC1020:
CC1021:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor.
H Jacques Garrigues,Yelena E Rubinchikova,C Michael Dipersio,Timothy M Rose
Journal of virology null
V M Belkin et al.
Biochemistry and molecular biology international, 40(1), 53-60 (1996-09-01)
Integrins of the beta 1 family were isolated from human smooth muscle. SDS-PAGE analysis and subsequent immunoblotting demonstrated that integrin samples contain a protein immunologically related to beta 1 integrin subunit with the previously undescribed apparent molecular mass 205 kD.
V M Belkin et al.
The Journal of cell biology, 111(5 Pt 1), 2159-2170 (1990-11-01)
A membrane glycoprotein complex was isolated and purified from human smooth muscle by detergent solubilization and affinity chromatography on collagen-Sepharose. The complex was identified as VLA-1 integrin and consisted of two subunits of 195 and 130 kD in SDS-PAGE. Liposomes
Regulation of trophoblast beta1-integrin expression by contact with endothelial cells
Thirkill, TL et al.
Cell communication and signaling : CCS, 2(1), 4-4 (2004)

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