CBL2117

Anti-Interleukin-6 Antibody, clone B-E8

clone B-E8, Chemicon^®, from mouse

• Synonym(s): IL-6, B-cell stimulatory factor 2, Interferon beta-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100; 300
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: B-E8, monoclonal
• species reactivity: human
• should not react with: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; neutralization: suitable
• isotype: IgG1
• NCBI accession no.: NM_000600.2
• UniProt accession no.: P05231
• shipped in: wet ice
• Gene Information: human ... IL6(3569)

Description

Specificity

Recognizes natural and recombinant forms of human Interleukin-6 with an affinity of 0.5 nM. Inhibits IL-6 binding to its receptors and blocks Interleukin-6 induced proliferation on B9 cells.

Immunogen

Recombinant human IL-6.

Application

Blocks Interleukin-6 induced proliferation on B9 cells: 20pg of antibody neutralizes the activity of 1U of IL-6. It is recommended that the sodium azide is removed from the antibody prior to use in this application.

Immunohistochemistry on frozen sections

Optimal working dilutions must be determined by the end user.

Detect Interleukin-6 using this Anti-Interleukin-6 Antibody, clone B-E8 validated for use in ELISA, FC, IH, NEUT.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Linkage

Replaces: 04-1095

Physical form

Format: Purified

The monoclonal is purified from tissue culture supernatant by ion exchange chromatography and is presented as a liquid in phosphate buffered saline, pH 7.2.

Storage and Stability

Store at 2-8°C for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable