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ABT1340

Sigma-Aldrich

Anti-Myosin-10/MYH10/Myosin IIB Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain Iib, MHC IIB

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

NCBI accession no.

NP_005955

UniProt accession no.

P35580

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYH10(4628)

General description

Myosin-10 (UniProt P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb) is encoded by the MYH10 (also known as NMMHCB) gene (Gene ID 4628) in human. Nonmuscle myosin II plays an important role in cell migration and invasion. The myosin II holoenzyme consists of two myosin heavy chains (MHCs), two essential light chains, and two regulatory light chains. Three different nonmuscle MHC II proteins exist in mammals, IIA (MYH9), IIB (MYH10), and IIC (MYH14), with both unique and overlapping roles. One of the initial steps in cancer cell metastasis is thought to be the process of epithelial–mesenchymal transition (EMT). Myosin IIB expression and myosin IIA heavy chain phosphorylation are upregulated upon TGF-β–induced EMT in mammary epithelial cells, while inhibition of myosin IIB expression in post-EMT mesenchymal cells reduces transmigration and invasion.

Specificity

Expected to react with all five spliced isoforms of human Myosin-10 reported by UniProt (P35580).

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human myosin-10.

Application

Anti-Myosin-10/MYH10/Myosin IIB Antibody is an antibody against Myosin-10/MYH10/Myosin IIB for use in Western Blotting, Immunocytochemistry, Immunofluorescence.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myosin-10/MYH10/Myosin IIB in 10 µg of MCF-10A cell lysate.
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.

Target description

~250 kDa observed. 229.0 kDa (isoform 1), 230.8 kDa (isoform 2), 231.4 kDa (isoform 3), 232.5 kDa (isoform 4), 230.0 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABT1340:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu Chen et al.
Nature methods, 18(5), 528-541 (2021-05-05)
Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro, posing challenges to their therapeutic use. There is an urgent need to advance strategies that ensure safe and robust long-term growth

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