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ABN332

Sigma-Aldrich

Anti-BICD2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Bicaudal D homolog 2, Bic-D 2, BICD2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BICD2(23299)

General description

BICD2 is the second human homolog discovered to the Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. Similar to the highly homologous protein BICD1, BICD2 can bind to dynein-dynactin complex, primarily through the dynamitin subunit of dynactin. The C-terminus of BICD2 targets the protein to the Golgi complex while the N-terminal domain of BICD2 co-immunoprecipitates with cytoplasmic dynein, suggesting BICD2 plays a role in the dynein-dynactin interaction on the surface of membranous organelles. Mice engineered to overexpress the BICD2 amino terminal domain in neurons developed amyotrophic lateral sclerosis (ALS)-like features such as Golgi fragmentation, neurofilament swelling in proximal axons, etc., suggesting that impaired dynein/dynactin function may explain some of the pathological features observed in ALS patients.

Specificity

This antibody will not cross-react with BICD1.

Immunogen

KLH-conjugated linear peptide corresponding to human BICD2.

Application

Anti-BICD2 detects levels of BICD2 proteins & has been published & validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected BICD2 in human cerebellum tissue.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BICD2 in 10 µg/mL of human lung tissue lysate.

Target description

~100 kDa observed. Uniprot describes two isoforms at ~94 kDa and ~97 kDa The calculated molecular weight is 94 kDa, however BICD2 has been shown as a ~100 kDa band in western blots. (Daniele, T., et al. (2011). Traffic. 12(7):902-911.) An uncharacterized band may appear at ~35 kDa in some lysates.

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABN332:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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