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Key Documents

Safety Information

ABN1386

Sigma-Aldrich

Anti-HES-1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Class B basic helix-loop-helix protein 39, bHLHb39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, hHL

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology), monkey (based on 100% sequence homology), hamster (based on 100% sequence homology), horse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_032261

UniProt accession no.

P35428

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HES1(3280)
mouse ... Hes1(15205)

General description

Transcription factor HES-1 (HES-1) also known as Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Immunogen

KLH-conjugated linear peptide corresponding to the near N-terminal of Mouse HES-1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected HES-1 in mouse hindbrain, cerebellum and kidney tissue.
This Anti-HES-1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of HES-1.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Hes-1 in 10 µg of mouse liver tissue lysate.

Target description

~30 kDa observed. Uncharacterized bands maybe observed in some lysates.

Linkage

Replaces: AB15470

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABN1386:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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