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ABE327

Sigma-Aldrich

Anti-Histone H2A Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Histone H2A type 1, H2A.1, Histone H2A/p

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_003500

UniProt accession no.

P0C0S8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HIST1H2AG(8969)

General description

Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

Specificity

Broad species reactivity expected based on sequence similarity.
This antibody recognizes Histone H2A at the C-terminus.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.

Application

Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.
Use Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.

Quality

Evaluated by Western Blot in HeLa acid extract and recombinant histone proteins.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.

Target description

~16 kDa observed

Analysis Note

Control
HeLa acid extract and recombinant histone proteins

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABE327:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaoyu Li et al.
EMBO reports, 16(9), 1192-1202 (2015-07-24)
In the absence of pathogen attack, organisms usually suppress immune responses to reduce the negative effects of disease resistance. Monoubiquitination of histone variants at specific gene loci is crucial for gene expression, but its involvement in the regulation of plant
Donghang Cheng et al.
The Journal of biological chemistry, 295(50), 17060-17070 (2020-10-04)
CARM1 is a protein arginine methyltransferase (PRMT) that acts as a coactivator in a number of transcriptional programs. CARM1 orchestrates this coactivator activity in part by depositing the H3R17me2a histone mark in the vicinity of gene promoters that it regulates.
Siwei Ouyang et al.
Neurobiology of aging, 36(10), 2850-2860 (2015-08-11)
The progressive loss of neurons causes neurodegenerative diseases. Because the accumulation of DNA breaks results in neuronal apoptosis, the lack of a variety of DNA damage repair-related proteins contributes to neurodegeneration. The ubiquitin ligase RNF8 plays an important role in
Xia Ting et al.
Nucleic acids research, 47(18), 9721-9740 (2019-09-11)
How chromatin dynamics is regulated to ensure efficient DNA repair remains to be understood. Here, we report that the ubiquitin-specific protease USP11 acts as a histone deubiquitinase to catalyze H2AK119 and H2BK120 deubiquitination. We showed that USP11 is physically associated

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