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Safety Information

ABC945

Sigma-Aldrich

Anti-GMPR Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

GMP reductase 1, Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GMPR(2766)

General description

GMP reductase 1 (EC 1.7.1.7; UniProt P36959; also known as Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1) is encoded by the GMPR (also known as GMPR1) gene (Gene ID 2766) in human. De novo biosynthesis of Guanosine 5′-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPR is shown to reduce intracellular GTP pools, thereby downregulating the level of several GTP-bound (active) RHO-GTPases that are essential for invadopodia formation, extracellular matrix degradation and invasion of melanoma cells both in cultures in vitro and in tumor xenografts in vivo. Consistent with its role as a negative regulator of the invasive melanoma phenotype, GMPR expression is reported to be either undetectable or drastically reduced in human melanoma cell lines when compared to normal melanocytes.

Specificity

This polyclonal antibody detects human GMP reductase 1 (GMPR) and stained predominantly the cytoplasm of cells in human tissue sections by immunohistochemistry.

Immunogen

Epitope: Between the second and third GMP-binding region.
KLH-conjugated linear peptide corresponding a sequence between the second and third GMP-binding region of human GMPR.

Application

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected GMPR in human prostate cancer, as well as in normal testis and skeletal muscle tissue sections.
Research Category
Apoptosis & Cancer
Research Sub Category
Cytoskeletal Signaling
This Anti-GMPR Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GMPR.

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GMPR in 10 µg of human skeletal muscle tissue lysate.

Target description

~35 kDa observed. 37.42 kDa calculated.

Physical form

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABC945:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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