Skip to Content
Merck
All Photos(2)

Key Documents

Safety Information

ABC60

Sigma-Aldrich

Anti-Endostatin Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Collagen alpha-1(XVIII) chain, Endostatin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
polyclonal
application:
IHC
WB
species reactivity:
rat, human, mouse
technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
citations:
1

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL18A1(80781)
mouse ... Col18A1(12822)

General description

Endostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis.

Specificity

This antibody recognizes Endostatin at the nonhelical region 11 domain.

Immunogen

Epitope: Nonhelical Region 11 Domain
Linear peptide corresponding to the nonhelical region 11 of mouse Endostatin.

Application

Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate.

Target description

~28 kDa observed.
A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832).

Linkage

Replaces: AB1880

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABC60:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service