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Key Documents

Safety Information

AB9304

Sigma-Aldrich

Anti-NAP-22 Antibody, complete sequence

Chemicon®, from rabbit

Synonym(s):

Neuronal acidic protein 22, CAP-23, BASP1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

NM_006317.3

UniProt accession no.

P80723

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Basp1(64160)

Specificity

Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].

Immunogen

Epitope: Complete sequence
Recombinant full length rat NAP-22.

Application

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Neuroregenerative Medicine
This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.

Physical form

Format: Purified
Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB9304:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T Laux et al.
The Journal of cell biology, 149(7), 1455-1472 (2000-06-28)
The dynamic properties of the cell cortex and its actin cytoskeleton determine important aspects of cell behavior and are a major target of cell regulation. GAP43, myristoylated alanine-rich C kinase substrate (MARCKS), and CAP23 (GMC) are locally abundant, plasmalemma-associated PKC
D Frey et al.
The Journal of cell biology, 149(7), 1443-1454 (2000-06-28)
CAP23 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. Overexpression of CAP23 in adult neurons of transgenic mice

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