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Key Documents

Safety Information

AB9260

Sigma-Aldrich

Anti-Ki-67 Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-Ki-67

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MKI67(4288)
mouse ... Mki67(17345)
rat ... Mki67(291234)

General description

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specificity

Recognizes Ki-67

Immunogen

Synthetic peptide from human Ki-67

Application

Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.
Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).

Western Blot Analysis:
A previous lot of this antibody was used in western blot.

Optimal working dilutions must be determined by the end user.

Quality

Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target description

Predicted: 359 kDa MW may be observed at ~345 or 395 kDa

Physical form

Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.

Analysis Note

Control
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB9260:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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