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Key Documents

Safety Information

AB755P

Sigma-Aldrich

Anti-Rat Collagen Type I Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-CAFYD, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable

isotype

IgG

suitability

not suitable for Western blot

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

General description

Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Specificity

Recognizes Rat Collagen Type I
The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Immunogen

Collagen Type I extracted and purified from rat skin.

Application

Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in RIA.

Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.

Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.

Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.

Quality

Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.

IHC-Paraffin Staining With Epitope Retrieval: Rat Skin

Physical form

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.

Analysis Note

Control
Rat hepatic stellate cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB755P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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CCN3/CCN2 regulation and the fibrosis of diabetic renal disease.
Riser BL, Najmabadi F, Perbal B, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC, Peterson DR
Journal of Cell Communication and Signaling null
Thy-1 expression by cardiac fibroblasts: lack of association with myofibroblast contractile markers.
Francois Hudon-David,Fatiha Bouzeghrane,Patrick Couture,Gaetan Thibault
Journal of Molecular and Cellular Cardiology null
Intraglomerular pressure and mesangial stretching stimulate extracellular matrix formation in the rat.
Riser, B L, et al.
The Journal of Clinical Investigation, 90, 1932-1943 (1992)
Partial hepatectomy-induced regeneration accelerates reversion of liver fibrosis involving participation of hepatic stellate cells.
Suarez-Cuenca, Juan A, et al.
Exp. Biol. Med, 233, 827-839 (2008)
Alan S Liu et al.
Scientific reports, 6, 33919-33919 (2016-09-28)
The biomechanical behavior of tissues under mechanical stimulation is critically important to physiological function. We report a combined experimental and modeling study of bioengineered 3D smooth muscle microtissues that reveals a previously unappreciated interaction between active cell mechanics and the
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