AB5454

Anti-TUC-4 Protein Antibody

serum, Chemicon^®

• Synonym(s): ULIP-1 Protein, CRMP-4, DRP-3, Dihydropyrimidinase-like 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: feline, monkey, human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_001387.2
• UniProt accession no.: Q14195
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... DPYSL3(1809)

Description

General description

TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997).

Specificity

Specifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia.

Immunogen

Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK)

Application

Anti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB.

Immunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection).
Immunocytochemistry: 1:1,000-1:5,000 (DAB detection).
Western blot: 1:2,500-1:25,000 using alkaline phosphatase.
Immunoprecipitation

Optimal working dilutions must be determined by end user.

Target description

64 & 74 kDa

Physical form

Rabbit Serum. Liquid containing 0.2% sodium azide.

Analysis Note

Control
POSITIVE CONTROL: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable