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Key Documents

Safety Information

AB5338P

Sigma-Aldrich

Anti-Cocaine and Amphetamine Related Transcript Antibody

Chemicon®, from rabbit

Synonym(s):

CART/CARP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_004291.2

UniProt accession no.

Q16568

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Cartpt(27220)
rat ... Cartpt(29131)

Specificity

Recognizes Cocaine- and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Immunogen

Mouse/rat CART peptide (55-102).

Application

Research Category
Neuroscience
Research Sub Category
CNS Control of Metabolism

Reward & Addiction
This Anti-Cocaine & Amphetamine Related Transcript Antibody is validated for use in ELISA, WB, IH for the detection of Cocaine & Amphetamine Related Transcript.
Western blotting: 1-10 μg/mL using ECL.

Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.

ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. No preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB5338P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B Meister
Vitamins and hormones, 59, 265-304 (2000-03-14)
Food intake is regulated via neural circuits located in the hypothalamus. During the past decade our knowledge on the specific mediators and neuronal networks that regulate food intake and body weight has increased dramatically. An important contribution to the understanding
P Kristensen et al.
Nature, 393(6680), 72-76 (1998-05-20)
The mammalian hypothalamus strongly influences ingestive behaviour through several different signalling molecules and receptor systems. Here we show that CART (cocaine- and amphetamine-regulated transcript), a brain-located peptide, is a satiety factor and is closely associated with the actions of two
J Douglass et al.
Gene, 169(2), 241-245 (1996-03-09)
PCR differential display screening has recently identified a rat mRNA termed CART (cocaine- and amphetamine-regulated transcript) which is transcriptionally regulated in the striatum following acute administration of psychomotor stimulants. The endogenous CART transcript is expressed in diverse rat brain structures
J Douglass et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 15(3 Pt 2), 2471-2481 (1995-03-01)
Neuronal plasticity associated with both short- and long-term administration of psychomotor stimulants involves alterations in specific patterns of gene expression. In order to screen for brain region specific mRNAs which are transcriptionally regulated by acute cocaine and amphetamine, PCR differential

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