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Safety Information

AB3562P

Sigma-Aldrich

Anti-Na+/K+/Cl- Cotransporter 2 Antibody

Chemicon®, from rabbit

Synonym(s):

NKCC2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_000338.2

UniProt accession no.

Q13621

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC12A1(6557)

Specificity

Recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC.

SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.

Immunogen

A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (Haas et al. 1987; Gamba et al. 1994; Simon et al. 1996; Mount et al. 1995; Igarashi et al. 1995; Mount et al. 1998). The immunogen peptide is available (catalog number AG205) for use in pre-absortion controls.

Application

Anti-Na+/K+/Cl-Cotransporter 2 Antibody detects level of Na+/K+/Cl-Cotransporter 2 & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL. Full length human NKCC2 is ~150 kDa.



ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB3562P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dendritic compartmentalization of chloride cotransporters underlies directional responses of starburst amacrine cells in retina.
Gavrikov, KE; Nilson, JE; Dmitriev, AV; Zucker, CL; Mangel, SC
Proceedings of the National Academy of Sciences of the USA null
Determination of uromodulin in human urine: influence of storage and processing.
Youhanna, S; Weber, J; Beaujean, V; Glaudemans, B; Sobek, J; Devuyst, O
Nephrology, Dialysis, and Transplantation null
Ting Li et al.
Molecular medicine reports, 17(6), 7618-7626 (2018-04-06)
Acute kidney injury (AKI) is one of the most common complications of sepsis. The roles of autophagy in AKI have been demonstrated in previous studies. Sequestosome‑1 (p62) has been demonstrated to serve essential roles in autophagy. The dysregulation of autophagy
Katharina Gerl et al.
The American journal of pathology, 187(7), 1496-1511 (2017-05-21)
Intrauterine hypoxia is a reason for impaired kidney development. The cellular and molecular pathways along which hypoxia exerts effects on nephrogenesis are not well understood. They are likely triggered by hypoxia-inducible transcription factors (HIFs), and their effects appear to be
Agnieszka Konopacka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(13), 5144-5155 (2015-04-04)
The Na-K-2Cl cotransporter 2 (NKCC2) was thought to be kidney specific. Here we show expression in the brain hypothalamo-neurohypophyseal system (HNS), wherein upregulation follows osmotic stress. The HNS controls osmotic stability through the synthesis and release of the neuropeptide hormone
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