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Key Documents

Safety Information

AB3269P

Sigma-Aldrich

Anti-Adiponectin Antibody

Chemicon®, from rabbit

Synonym(s):

Acrp30, AdipoQ, apM1, GBP28

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_004797.2

UniProt accession no.

Q15848

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADIPOQ(9370)

Specificity

Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.

SPECIES REACTIVITIES:

There is weak reactivity with rat. Reactivity with other species has not been confirmed.

Immunogen

Recombinant mouse adiponectin from E. coli.

Application

Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:5,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: MABS176

Physical form

Format: Purified
Purified immunoglobulin in PBS, pH 7.4.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB3269P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adiponectin opposes endothelin-1-mediated vasoconstriction in the perfused rat hindlimb.
Bussey, CT; Kolka, CM; Rattigan, S; Richards, SM
American Journal of Physiology. Heart and Circulatory Physiology null
Jiung-Pang Huang et al.
Journal of molecular endocrinology, 60(4), 307-321 (2018-03-28)
Many studies have reported the causes of obese metabolic syndrome (MS); however, the causes of nonobese MS (NMS) remain unknown. In this study, we demonstrated that inflamed dysfunctional adipose tissue plays a crucial role in cholesterol-induced NMS. Control (C), high
Ruben Garcia-Martin et al.
Cell reports, 38(3), 110277-110277 (2022-01-20)
Exosomes/small extracellular vesicles (sEVs) can serve as multifactorial mediators of cell-to-cell communication through their miRNA and protein cargo. Quantitative proteomic analysis of five cell lines representing metabolically important tissues reveals that each cell type has a unique sEV proteome. While

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