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Key Documents

Safety Information

AB3244

Sigma-Aldrich

Anti-GLP-1 (7-36) Antibody, CT amide-reactive

serum, Chemicon®

Synonym(s):

Glucagon-like peptide-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

should not react with

guinea pig

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GCG(2641)

Specificity

Glucagon-like peptide-1(7-36) amide (GLP-1), C-terminal reactive. Cross-reacts fully with GLP-1(1-36) amide but shows <0.1% cross reactivity with the unamidated forms GLP-1(7-37) and GLP-1(1-37) or other related peptides such as hGLP-2, glucagon, hGIP and VIP.

SPECIES REACTIVITIES: Most mammalian species. GLP-1 is identical in all mammalian species studied except guinea pig and presumably its South American rodent relatives.

Immunogen

Epitope: C-terminus amide-reactive
Whole synthetic peptide.

Application

Anti-GLP-1 (7-36) Antibody, C-terminus amide-reactive detects level of GLP-1 (7-36) & has been published & validated for use in ELISA, RIA.
RIA: 1:300,000. Ka = 0.8 x 10E12 l/mol for GLP-1(7-36) amide. Detection limit 0.1 fmol/tube, 95% confidence limits.

EIA (capture). Detection limit 1.5 pmol/liter.

Note: Plasma samples require extraction for RIA, but not for sandwich EIA. Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Metabolic Hormones & Receptors

Physical form

Rabbit serum, diluted. Liquid in PBS containing 0.05% sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB3244:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C Pelegri et al.
Molecular medicine (Cambridge, Mass.), 7(5), 311-319 (2001-07-28)
Glucagon-producing alpha cells play a crucial role during the perinatal period. Because of their peri-islet localization near the early dendritic and macrophage cell infiltration, we thought it pertinent to investigate alpha cells in greater depth in nonobese diabetic (NOD) mice

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