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Safety Information

AB3085

Sigma-Aldrich

Anti-Na+/H+ Exchanger-3 Antibody

Chemicon®, from rabbit

Synonym(s):

NHE3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NP_004165

UniProt accession no.

P48764

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Slc9A3(24784)

Specificity

Recognizes NHE3.
The immunogen sequence shares 90% homology with mouse NHE3.

Immunogen

A 22 AA peptide within the cytoplasmic, c-terminal domain of the rat NHE3 (Brant et al. 1995; Orlowsji et al. 1992; Chris Yun et al. 1995; Josette & Pouyssegur 1995). Control peptide is available, cat# AG779.

Application

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Na+/H+ Exchanger-3 Antibody is validated for use in ELISA, WB for the detection of Na+/H+ Exchanger-3.
Western blot: 1-10 μg/mL using Chemiluminescence technique. A band of approximately 90 kD has been detected in brush border membrane (Yohsioka et al. 1997). ELISA: 1:100,000 using 50-100 ng control peptide/well.

Optimal working dilutions must be determined by end user.

Target description

93 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified

Storage and Stability

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Kidney, jejunum, colon, and stomach tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB3085:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jeffrey Weiss et al.
Endocrinology, 149(12), 6198-6206 (2008-08-23)
The estrogen receptor-alpha (ERalpha) acts through multiple pathways, including estrogen response element (ERE)-dependent (classical) and ERE-independent (nonclassical) mechanisms. We previously created a mouse model harboring a two-amino-acid mutation of the DNA-binding domain (E207A, G208A) that precludes direct binding of ERalpha
Differential traffic of proximal tubule Na+ transporters during hypertension or PTH: NHE3 to base of microvilli vs. NaPi2 to endosomes.
Yang, LE; Maunsbach, AB; Leong, PK; McDonough, AA
American Journal of Physiology: Renal Physiology null
Alena Cherezova et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(2), 2156-2170 (2018-09-27)
cAMP is a universal second messenger regulating a plethora of processes in the kidney. Two downstream effectors of cAMP are PKA and exchange protein directly activated by cAMP (Epac), which, unlike PKA, is often linked to elevation of [Ca2+]i. While
Evidence for NHE3-mediated Na transport in sheep and bovine forestomach.
Rabbani, I; Siegling-Vlitakis, C; Noci, B; Martens, H
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
Phenol injury-induced hypertension stimulates proximal tubule Na+/H+ exchanger activity.
Leong, PK; Yang, LE; Landon, CS; McDonough, AA; Yip, KP
American Journal of Physiology: Renal Physiology null
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