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Key Documents

Safety Information

AB10521

Sigma-Aldrich

Anti-Neuropilin-1 Antibody

serum, from rabbit

Synonym(s):

CD304 antigen, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor, Npn 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

species reactivity (predicted by homology)

human (94%)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_659566.1

UniProt accession no.

Q9QWJ9

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NRP1(8829)

General description

Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neuropilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. It is expected to form complexes with the plexinA subfamily members and mediate the semaphorin-elicited inhibitory signals into neurons. Third, in endothelial cells, neuropilin-1 binds a potent endothelial cell mitogen, vascular endothelial growth factor (VEGF)165, and regulates vessel formation.

Specificity

This antibody recognizes Neuropilin-1.

Immunogen

Epitope: Unknown
This antibody is generated from rabbits immunized with a His-tagged partial recombinant rat Neuropilin-1.

Application

Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
This Anti-Neuropilin-1 Antibody is validated for use in WB, IH, IP, IF for the detection of Neuropilin-1.

Quality

Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: : 1:1,000 dilution of this antibody detected Neuropilin 1 on 10 µg of PC12 cell lysate.

Target description

130 kDa

Physical form

Rabbit serum in buffer containing 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PC12 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB10521:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R J Giger et al.
Neuron, 21(5), 1079-1092 (1998-12-18)
Neuropilins bind secreted members of the semaphorin family of proteins. Neuropilin-1 is a receptor for Sema III. Here, we show that neuropilin-2 is a receptor for the secreted semaphorin Sema IV and acts selectively to mediate repulsive guidance events in
A L Kolodkin et al.
Cell, 90(4), 753-762 (1997-08-22)
The semaphorin family contains a large number of phylogenetically conserved proteins and includes several members that have been shown to function in repulsive axon guidance. Semaphorin III (Sema III) is a secreted protein that in vitro causes neuronal growth cone

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