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Key Documents

Safety Information

AB1033

Sigma-Aldrich

Anti-Versican Antibody, a.a. 1360-1439 of mouse versican

Chemicon®, from rabbit

Synonym(s):

GAG beta Domain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_001135.2
NM_013227.2

UniProt accession no.

P16112

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

General description

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

Specificity

Mouse versican GAG beta domain.

Immunogen

Epitope: a.a. 1360-1439 of mouse versican
GST fusion protein containing amino acids 1360-1439 of mouse versican.

Application

Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P).
Immunohistochemistry (formally fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining. {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.

Western blotting: 0.5 μg/mL

Immunoprecipitation:

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
The immunoglobulin was affinity purified against the recombinant antigen and is presented in PBS. Anti-GST reactivity has not been adsorbed. Liquid in PBS.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeat freeze/thaw cycles.

Analysis Note

Control
Immunohistochemistry Positive control: surrounding cartilageWestern blotting positive control: total 13 day mouse embryo

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB1033:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Design of a miniature tissue culture system to culture mouse heart valves.
Lieber SC, Kruithof BP, Aubry N, Vatner SF, Gaussin V
Annals of Biomedical Engineering null
Proteolytic cleavage of versican and involvement of ADAMTS-1 in VEGF-A/VPF-induced pathological angiogenesis.
Fu, Y; Nagy, JA; Brown, LF; Shih, SC; Johnson, PY; Chan, CK; Dvorak, HF; Wight, TN
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Fabienne Meier-Abt et al.
Breast cancer research : BCR, 15(2), R36-R36 (2013-04-30)
Early pregnancy has a strong protective effect against breast cancer in humans and rodents, but the underlying mechanism is unknown. Because breast cancers are thought to arise from specific cell subpopulations of mammary epithelia, we studied the effect of parity
Angiotensin II increases vascular proteoglycan content preceding and contributing to atherosclerosis development.
Huang, F; Thompson, JC; Wilson, PG; Aung, HH; Rutledge, JC; Tannock, LR
Journal of Lipid Research null
Early but not late pregnancy induces lifelong reductions in the proportion of mammary progesterone sensing cells and epithelial Wnt signaling.
Meier-Abt, F; Brinkhaus, H; Bentires-Alj, M
Breast Cancer Research null
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