69079

Perfect Protein Markers, 10-225 kDa

• Synonym(s): protein markers, protein molecular weight markers, protein standards
• UNSPSC Code: 41105337
• NACRES: NC.01

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).

The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).

The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).

The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.

Features and Benefits

• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining

• Recombinant, unmodified markers producesharp, accurately sized bands

• Available in two size ranges:

15-150 kDa

10-225 kDa

Warning

Toxicity: Toxic (F)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable